WebThe volume of the 50 mg/mL ampicillin stock solution needed to be added to 500 mL of bacterial growth medium to achieve a final concentration ... Calculate the volume in mL … http://www.protocol-online.org/biology-forums/posts/36393.html

Addgene: Protocol - How to Inoculate a Bacterial Culture

WebThe final concentration for LB liquid culture is 50 ug/ml. To obtain this in 100ml (the amount in each LB bottle), add 100ul of 50 mg/ml stock solution OR 50ul of 100 mg/ml stock … Web9 feb. 2008 · Stock solutions made in 50% alcohol remain liquid at -20°C and are easy to pipet. Cool agar to 55°C or below prior to adding antibiotic. Ampicillin available from … free tumblr themes with infinite scroll

How can i prepare ampicillin and kanamycin stock solution?

Web&Company. Plates were prepared by mixing 1 ml antimicrobial solution with 19 mlmoltenDSTagar (Oxoid)at atempratureofless than50°C. Thefinal concentrations of the drug in agar were 2, 4, 6, 8, 10, and20 Ztg/ml for ampicillin and cephaloridine and 0-25, 0-5, 1, 2, and 4 ug/ml for trimethoprim andgentamicin.Approximately0-003mlofa4-hour Web13 apr. 2024 · Among these Fusarium-derived 1,4-naphthoquinone analogs (109–115), compound 109 showed potent anti-Gram-positive bacteria activity against B. cereus and S. pyogenes with MIC of <1 µg/mL and anti-C. albicans activity with IC 50 (the half maximal inhibitory concentration) of 6.16 µg/mL , and 110–115 demonstrated moderate inhibitory … Webclone was inoculated in 1 mL LB containing either ampicillin (100 µg/ mL) or chloramphenicol (34 µg/mL) depending on the MGC vector. A BP recombinational reaction contains 2 µL of 5 × BP3 buffer; 2 µL of BP clonase; 1 µL of pDONR223 (150 ng/µL); 2 µL of PCR product (2-200 ng/µL); 3 µL H 2 O. farzy gingerbread house