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Lamp2a sirna

TīmeklisSynthetic peptide corresponding to Human LAMP2A aa 350 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin. LAMP2 has 3 distinct isoforms, LAMP2A, 2B & 2C. Database link: P13473 (Peptide available as ab23322) 阳性对照 WB: Human liver tissue lysate. ICC: CaCo2 cells, HeLa, MCF7 and HepG2 cells. TīmeklisSynthetic peptide corresponding to Human LAMP2A aa 350 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin. LAMP2 has 3 distinct isoforms, …

Up-Regulated Expression of LAMP2 and Autophagy Activity during ... - PubMed

Tīmeklis2024. gada 28. marts · SciAdv丨LAMP2A介导了蛋白质向外泌体的分选和包装. 研究 2024-03-28 20:36 来源：已关闭评论 1. 外泌体是内体来源的细胞外囊泡，可以在不同细胞、组织或器官之间转移脂质、RNA 和蛋白质。. 来自葡萄牙里斯本大学的研究人员发现，含有KFERQ五肽序列的蛋白质可以通过 ... Tīmeklis2024. gada 18. febr. · Forty-eight hours post siRNA transfection, the expression of Flag-TDP-43 WT was evaluated at by Western blot at indicated time points. (C) Densitometric quantification of Flag-TDP-43 WT from (B). (D) Lamp2A siRNA was transfected in HEK293 Flag-TDP-12xQ/N cell line as indicated in (B). The expression of Flag-TDP … texpool.org https://msink.net

Silencing of Lamp2a promotes SARS-CoV-2 infection in

Tīmeklis2024. gada 25. marts · LAMP2A is required for the sorting of selected proteins into extracellular vesicles To investigate the impact of LAMP2A in exosome protein cargo, we used the CRISPR-Cas9 system to … Tīmeklis2024. gada 7. febr. · Lamp2 siRNA in normal conditions caused the injury of lysosomal membranes and the decline of cardiomyocytes viability. These results indicated that Lamp2 is involved in the regulation of LMP and cell death and confers cardiomyocytes resistance against OGD treatment. Tīmeklis2024. gada 7. nov. · LAMP2A possesses a highly glycosylated luminal region that is common to all LAMP2 isoforms and a specific 11-amino-acid-long cytosolic C … texportal holterbosch

SignalSilence® AMPKα2 siRNA II Cell Signaling Technology

Category:细胞溶酶体降解A型流感病毒HA蛋白的机制研究_参考网

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Lamp2a sirna

LAMP2 siRNAs - Thermo Fisher Scientific

Tīmeklis1.1 主要实验材料 人胚胎肾细胞293T 及Atg3 基因敲除的293T 细胞、质粒pXBP1u-Luc、pCS-H5HA由本实验室保存；GFP-250 质粒由Elizabeth Sztul 惠赠；表达伪狂犬病毒(PRV) gE 蛋白的重组质粒PRV-gE 及鼠源抗gE 蛋白单克隆抗体(MAb)由田志军研究员惠赠；Lamp2a siRNA 购自GenePharma 公司 ... Tīmeklis2024. gada 24. marts · a Western blot analysis of LAMP-2A and N-CoR under different siRNA treatment conditions confirmed inhibitory effect of LAMP-2A siRNA (LAMP-2A siR) and N-CoR siRNA (N-CoR siR). Although LAMP-2A inhibition resulted in significant increase of N-CoR, addition of N-CoR siR almost eliminated this effect.

Lamp2a sirna

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Tīmeklis2024. gada 24. marts · LAMP-2A siRNA and shRNA were constructed to manipulate CMA activation. The role of CMA and downstream mechanism through degradation of N-CoR and arresting UPR mediated apoptosis were explored in GBM cells and nude mouse xenograft model. Results

Tīmeklis2012. gada 9. aug. · Both LAMP1 and LAMP2 are highly glycosylated, and are thought to have overlapping functions in tumor cell metastasis. 12-15 Levels of LAMP2A are controlled by the dynamic distribution of LAMP2A between the lumen of the lysosome and its membrane. 16 Autophagy acts as a double-edged sword. Tīmeklis2024. gada 7. nov. · LAMP2 expression in the retina and the RPE in young and aged mice. ( A) Western blot analyses of LAMP2 expression in the retina or the RPE/choroid from WT or Lamp2 KO mice. LAMP2 was abundantly expressed in the RPE/choroid of WT mice, but was completely absent in the RPE/choroid of Lamp2 KO mice.

Tīmeklis2012. gada 9. aug. · Breast cancer cells transfected with either LAMP2A or LAMP2A siRNA were used to investigate the effect of LAMP2A on modulating PCC of the cells in the presence of 150 nM H 2 O 2. PCC was reduced by 50–60% in T47D cells that harbor ectopic expression of LAMP2A when compared with the control pcDNA3 … TīmeklissiRNA. 48 h later, cells were lysed and assayed for ASYN and Lamp2a levels. Non transfected cells were used as a control (ctrl). Representative immunoblots of …

Tīmeklis2016. gada 14. sept. · On the other hand, LAMP2 knockdown by siRNA led to a marked blockage of autophagy, prevention of NE differentiation and decrease of cell survival. …

TīmeklisOur portfolio includes three types of siRNA processing and purification for in vivo RNAi research. in vivo-ready (IVR) —This process involves standard RNAi oligo synthesis followed by diafiltration to remove salts and solvents to a level <200 µS and sterile filtration, as well as endotoxin testing. sword fluteTīmeklisProduct Description SignalSilence ® AMPKα2 siRNA II from Cell Signaling Technology (CST) allows the researcher to specifically inhibit AMPKα2 expression using RNA … texport fire wildlandTīmeklis暨南大学,数字图书馆. 开馆时间：周一至周日7:00-22:30 周五 7:00-12:00; 我的图书馆 texpool portfolioTīmeklis2012. gada 7. febr. · Next, we examined whether lysosomal translocation of GAPDH-HT would occur in HeLa cells with siRNA-mediated knockdown of LAMP2A. We … sword flying destiny 2 consoleTīmeklis2024. gada 20. dec. · Then the substrate/chaperon complex binds the lysosome membrane-bound LAMP2A, which triggers the LAMP2A multimerization and followed lysosomal internalization of substrate proteins for degradation. The reduced neuronal CMA activity is observed in the aging brain and neurodegenerative diseases. texpool yieldsTīmeklis2024. gada 5. febr. · EGLN3 and LAMP2A siRNAs (50–75 nM) were transfected into cells using Lipofectamine 2000 reagent (Invitrogen) as described previously . siRNA transfection efficiency was verified by ... sword flying halo 2Tīmeklis2024. gada 21. marts · LAMP2 (Lysosomal Associated Membrane Protein 2) is a Protein Coding gene. Diseases associated with LAMP2 include Danon Disease and Left … texport airlock